Correlation between protein desorption behavior and its adsorption enthalpy change in polymer grafted anion exchange chromatography

Anion-Exchange Chromatography of Phosphopeptides: Weak Anion Exchange versus Strong Anion Exchange and Anion-Exchange Chromatography versus Electrostatic Repulsion–Hydrophilic Interaction Chromatography

Most phosphoproteomics experiments rely on prefractionation of tryptic digests before online liquid chromatography-mass spectrometry. This study compares the potential and limitations of electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) and anion-exchange chromatography (AEX). At a pH higher than 5, phosphopeptides have two negative charges per residue and are well-retained...

Shear-Induced Deformation and Desorption of Grafted Polymer Layers

We describe the behaviour of grafted polymer layers in strong solvent shear flows within a model where only a subset of chains are exposed to the flow (hence to the tension arising from hydrodynamic drag forces), leaving the remainder protected. We show that for quite small values of the shear rate, I, the system reaches a self-regulating state where the lowest possible fraction of grafted chai...

Preparation and Evaluation of Anion-Exchange Polymer

Binding site and elution behavior of DNA and other large biomolecules in monolithic anion-exchange chromatography.

Our previous study has shown that there is a good correlation between the number of charges of DNA (from trimer to 50-mer) and the number of binding sites B in electrostatic interaction chromatography (ion-exchange chromatography, IEC). It was also found that high salt (NaCl) concentration is needed to elute large DNAs (>0.6M). In this paper we further performed experiments with large DNAs (up ...

Anion-exchange displacement centrifugal partition chromatography.

Ion-exchange displacement chromatography has been adapted to centrifugal partition chromatography. The use of an ionic liquid, benzalkonium chloride, as a strong anion-exchanger has proven to be efficient for the preparative separation of phenolic acid regioisomers. Multigram quantities of a mixture of three hydroxycinnamic acid isomers were separated using iodide as a displacer. The displaceme...